Contact an Account ManagerDetermination of free and total silibinin diastereomers in plasma using RP chromatography with UV detection
Summary
Type: HPLC
Application #: 106970
Catalog#: 720040.30 - MN HPLC Column, Nucleosil C18, 120A, 3um, 3x125mm
Detection: UV, 288 nm
Conditions: eluent: 60:40 (v/v) mixture of methanol / 0.1 M phosphate buffer pH 3
flow rate 0.5ml/min
temperature 40 °C
After the analytes and the internal standard (naringenin) had been eluted, the mobile phase was changed to 100% methanol at 0.5 ml/min for 2 min followed by 21 min of equilibration at initial conditions.
Authors: Rickling B
Source: J Chromatogr B 670 (1995) 267-277
Details
Legend: Fig. 3. Chromatograms of total (free + conjungated) silibinin diastereomers.
(A) Plasma spiked with silibinin diastereomers at 200 ng/ml. (B) plasma spiked
with silibinin diastereomers at 12.5 ng/ml